Catalog Number - 40031-10P-2

40031-10P-2 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a unique mixed‑mode separation mechanism that goes beyond traditional reversed phase or ion exchange columns.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple retention interactions:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interactions)

This combination allows highly effective separation of basic, polar, ionizable, and structurally similar compounds, particularly in complex matrices where conventional columns struggle.

The TYPE‑C silica backbone adds significant performance advantages:

• Direct silicon–carbon bonding for increased durability

• Stability at very low pH (<2) without degradation

• Faster equilibration compared to conventional silica

• Reduced sensitivity to mobile phase moisture

100 mm Length – Balanced Resolution for LC‑MS Workflows

The 100 mm length is one of the most versatile formats, offering a balance between speed and resolving capability.

• Higher resolution than 50 mm columns

• Improved separation of closely related compounds

• Efficient run times for routine LC‑MS workflows

✅ Recommended for LC‑MS methods requiring improved resolution without long run times

Flow Rate & System Requirements (2.1 mm ID, 4 µm)

⚠️ Requires low‑flow capable HPLC or UHPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Moderate backpressure compared to sub‑2 µm columns

• Requires accurate low‑flow solvent delivery

• Optimized for low‑dispersion flow paths and small detector volumes

✅ Important: Standard HPLC systems must be capable of stable and precise low‑flow operation

✅ System Fit: Ideal for LC‑MS systems and modern analytical platforms

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting compounds

• Effective for positively charged and ionizable analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Retention can be tuned via pH and ionic strength

✅ Ideal when C18, phenyl, or traditional ion exchange columns fail

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under aggressive conditions including low pH

• Resistant to hydrolysis

Faster Equilibration

• Reduces overall analysis time

No Strict Moisture Control in HILIC

• Easier method development

Extended Column Lifetime

• Durable for demanding analytical workflows

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Balanced Performance (4 µm Particles)

• Lower pressure than UHPLC columns

• Compatible with a wider range of systems

LC‑MS Optimized

• Minimal salt required in HILIC mode

• Cleaner MS signals

Typical Applications

• LC‑MS method development

• Complex matrix separations

• Basic and positively charged compounds

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires low‑flow capable HPLC or UHPLC system

• Suitable for both hydrophilic and hydrophobic compounds

• pH control is critical for WCX functionality